Dataset Information
The Cell Cycle G2/M Block Is an Indicator of Cellular Radiosensitivity.
Ontology highlight
ABSTRACT: Background
Determination of the radiosensitivity of a specific tumor is essential to its precision tumor radiotherapy, but the measurement of cellular radiosensitivity with a routine colony forming assay is both labor- and time-consuming. An alternative option allowing rapid and precise prediction of radiosensitivity is necessary.Methods
In this study, we exposed 4 in vitro cultured cell lines to various doses of X-rays or carbon ions and then measured their radiosensitivities with a routine colony-forming assay, and monitored the kinetics of cell cycle distribution with routine propidium iodine staining and flow cytometry.Results
Based on the results, we correlated cellular radiosensitivity with a dynamic assay of cell cycle distribution, specifically, the negative correlation of cellular radiosensitivity with the accumulated G2/M arrested cells at 48 hours after exposure. The higher the proportion of accumulated G2/M arrested cells at 48 hours after exposure, the lower the radiosensitivity of the cell line, that is, the higher radioresistance of the cell line.Conclusion
These findings provide an optional application of regular cell cycle analysis for the prediction of tumor radiosensitivity.
SUBMITTER: Liu C
PROVIDER: S-EPMC6902394 | biostudies-literature | 2019 Oct-Dec
REPOSITORIES: biostudies-literature
Publications
The Cell Cycle G2/M Block Is an Indicator of Cellular Radiosensitivity.
Dose-response : a publication of International Hormesis Society 20191001 4
<h4>Background</h4>Determination of the radiosensitivity of a specific tumor is essential to its precision tumor radiotherapy, but the measurement of cellular radiosensitivity with a routine colony forming assay is both labor- and time-consuming. An alternative option allowing rapid and precise prediction of radiosensitivity is necessary.<h4>Methods</h4>In this study, we exposed 4 in vitro cultured cell lines to various doses of X-rays or carbon ions and then measured their radiosensitivities wi ...[more]
