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Single-molecule detection on a portable 3D-printed microscope.

ABSTRACT: Single-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and fluorescence correlation spectroscopy (FCS) in a simple format and is widely applicable to the detection of single fluorophores, proteins, liposomes or bacteria. The power of single-molecule detection is demonstrated by detecting single ?-synuclein amyloid fibrils, that are currently evaluated as biomarkers for Parkinson's disease, with an improved sensitivity of >100,000-fold over bulk measurements.

SUBMITTER: Brown JWP

PROVIDER: S-EPMC6906517 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Single-molecule detection on a portable 3D-printed microscope.

Brown James W P JWP Bauer Arnaud A Polinkovsky Mark E ME Bhumkar Akshay A Hunter Dominic J B DJB Gaus Katharina K Sierecki Emma E Gambin Yann Y

Nature communications 20191211 1

Single-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and ...[more]

PMID: 31827096

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