Ontology highlight
ABSTRACT:
SUBMITTER: Wu Y
PROVIDER: S-EPMC6909005 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
iScience 20191125
A major challenge in using human pluripotent stem cells (hPSCs) in therapy is the risk of teratoma formation due to contaminating undifferentiated stem cells. We used CRISPR-Cas9 for in-frame insertion of a suicide gene, iC9, into the endogenous SOX2 locus in human embryonic stem cell (ESC) line H1 for specific eradication of undifferentiated cells without affecting differentiated cells. This locus was chosen over NANOG and OCT4, two other well-characterized stem cell loci, due to significantly ...[more]