Unknown

Dataset Information

0

MiR-770-5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1.


ABSTRACT: Colon cancer is one of the most common types of cancer worldwide. Methotrexate (MTX) is a chemotherapy drug used for the treatment of multiple types of cancer, such as colon and breast cancer. To determine the effects of MTX treatment on colorectal adenocarcinoma cell lines, a microRNA (miRNA) microarray was used to detect miRNA expression profiles of HT-29 colorectal adenocarcinoma MTX-resistant cells and their parental cells. The results demonstrated that 641 genes and 43 miRNAs were differentially expressed between HT-29 MTX-sensitive cells and MTX-resistant cells. In addition, 12 miRNAs and their co-expressed genes were highly correlated in MTX treatment, and one of the identified miRNAs, miR-770-5p, was studied in subsequent experiments. Upregulation of miR-770-5p significantly decreased the sensitivity of HT-29 cells to MTX. Using bioinformatics software, homeodomain-interacting protein kinase 1 (HIPK1) was identified to be a putative target gene of miR-770-5p, which was confirmed by a luciferase reporter assay. Downregulation of miR-770-5p target gene HIPK1 significantly decreased the sensitivity of HT-29 cells to MTX. These results suggest that miR-770-5p may be involved in the regulation of colon cancer resistance to MTX by regulating the expression of the target gene HIPK1.

SUBMITTER: Zhang D 

PROVIDER: S-EPMC6909793 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

miR-770-5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1.

Zhang Dawei D   Li Ying Y   Sun Peilong P  

Experimental and therapeutic medicine 20191119 1


Colon cancer is one of the most common types of cancer worldwide. Methotrexate (MTX) is a chemotherapy drug used for the treatment of multiple types of cancer, such as colon and breast cancer. To determine the effects of MTX treatment on colorectal adenocarcinoma cell lines, a microRNA (miRNA) microarray was used to detect miRNA expression profiles of HT-29 colorectal adenocarcinoma MTX-resistant cells and their parental cells. The results demonstrated that 641 genes and 43 miRNAs were different  ...[more]

Similar Datasets

| S-EPMC7434570 | biostudies-literature
| S-EPMC4225484 | biostudies-literature
| S-EPMC5288183 | biostudies-literature
| S-EPMC7679243 | biostudies-literature
| S-EPMC4886683 | biostudies-other
| S-EPMC6476517 | biostudies-literature
| S-EPMC7189364 | biostudies-literature
| S-EPMC4615858 | biostudies-literature
| S-EPMC10149273 | biostudies-literature
| S-EPMC5883523 | biostudies-literature