PASTMUS: mapping functional elements at single amino acid resolution in human cells.

ABSTRACT: Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins-three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6913009 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature