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PASTMUS: mapping functional elements at single amino acid resolution in human cells.

ABSTRACT: Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins-three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.

SUBMITTER: Zhang X

PROVIDER: S-EPMC6913009 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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PASTMUS: mapping functional elements at single amino acid resolution in human cells.

Zhang Xinyi X Yue Di D Wang Yinan Y Zhou Yuexin Y Liu Ying Y Qiu Yeting Y Tian Feng F Yu Ying Y Zhou Zhuo Z Wei Wensheng W

Genome biology 20191216 1

Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biologi ...[more]

PMID: 31842968

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