Ontology highlight
ABSTRACT:
SUBMITTER: Thibault D
PROVIDER: S-EPMC6914776 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Thibault Derek D Jensen Paul A PA Wood Stephen S Qabar Christine C Clark Stacie S Shainheit Mara G MG Isberg Ralph R RR van Opijnen Tim T
Nature communications 20191216 1
While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individual transposon mutants into growth medium-in-oil droplets, thereby enabling isolated growth, free from the influence of the population. Here we describe and validate microfluidic chip design, producti ...[more]