Ontology highlight
ABSTRACT:
SUBMITTER: Keich U
PROVIDER: S-EPMC6919216 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Keich Uri U Tamura Kaipo K Noble William Stafford WS
Journal of proteome research 20190103 2
Decoy database search with target-decoy competition (TDC) provides an intuitive, easy-to-implement method for estimating the false discovery rate (FDR) associated with spectrum identifications from shotgun proteomics data. However, the procedure can yield different results for a fixed data set analyzed with different decoy databases, and this decoy-induced variability is particularly problematic for smaller FDR thresholds, data sets, or databases. The average TDC (aTDC) protocol combats this pro ...[more]