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Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages.


ABSTRACT: Escherichia coli ST131 is a clinical challenge due to its multidrug resistant profile and successful global spread. They are often associated with complicated infections, particularly urinary tract infections (UTIs). Bacteriocins play an important role to outcompete other microorganisms present in the human gut. Here, we characterized bacteriocin-encoding plasmids found in ST131 isolates of patients suffering from a UTI using both short- and long-read sequencing. Colicins Ia, Ib and E1, and microcin V, were identified among plasmids that also contained resistance and virulence genes. To investigate if the potential transmission range of the colicin E1 plasmid is influenced by the presence of a resistance gene, we constructed a strain containing a plasmid which had both the colicin E1 and blaCMY-2 genes. No difference in transmission range was found between transformant and wild-type strains. However, a statistically significantly difference was found in adhesion and invasion ability. Bacteriocin-producing isolates from both ST131 and non-ST131 lineages were able to inhibit the growth of other E. coli isolates, including other ST131. In summary, plasmids harboring bacteriocins give additional advantages for highly virulent and resistant ST131 isolates, improving the ability of these isolates to compete with other microbiota for a niche and thereby increasing the risk of infection.

SUBMITTER: da Cruz Campos AC 

PROVIDER: S-EPMC6920910 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Determining the Virulence Properties of <i>Escherichia coli</i> ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other <i>E. coli</i> Lineages.

da Cruz Campos Ana Carolina AC   Cavallo Francis M FM   L Andrade Nathália N   van Dijl Jan Maarten JM   Couto Natacha N   Zrimec Jan J   Lo Ten Foe Jerome R JR   P Rosa Ana C AC   V Damasco Paulo P   Friedrich Alex W AW   Chlebowicz-Flissikowska Monika A MA   Rossen John W A JWA  

Microorganisms 20191106 11


<i>Escherichia coli</i> ST131 is a clinical challenge due to its multidrug resistant profile and successful global spread. They are often associated with complicated infections, particularly urinary tract infections (UTIs). Bacteriocins play an important role to outcompete other microorganisms present in the human gut. Here, we characterized bacteriocin-encoding plasmids found in ST131 isolates of patients suffering from a UTI using both short- and long-read sequencing. Colicins Ia, Ib and E1, a  ...[more]

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