Dataset Information

Genetic and serologic surveillance of canine (CIV) and equine (EIV) influenza virus in Nuevo Leon State, Mexico.

ABSTRACT: Background:Despite the uncontrolled distribution of the Influenza A virus through wild birds, the detection of canine influenza virus and equine influenza virus in Mexico was absent until now. Recently, outbreaks of equine and canine influenza have been reported around the world; the virus spreads quickly among animals and there is potential for zoonotic transmission. Methods:Amplification of the Influenza A virus matrix gene from necropsies, nasal and conjunctival swabs from trash service horses and pets/stray dogs was performed through RT-PCR. The seroprevalence was carried out through Sandwich enzyme-linked immunosorbent assay system using the M1 recombinant protein and polyclonal antibodies anti-M1. Results:The matrix gene was amplified from 13 (19.11%) nasal swabs, two (2.94%) conjunctival swabs and five (7.35%) lung necropsies, giving a total of 20 (29.41%) positive samples in a pet dog population. A total of six (75%) positive samples of equine nasal swab were amplified. Sequence analysis showed 96-99% identity with sequences of Influenza A virus matrix gene present in H1N1, H1N2 and H3N2 subtypes. The phylogenetic analysis of the sequences revealed higher identity with matrix gene sequences detected from zoonotic isolates of subtype H1N1/2009. The detection of anti-M1 antibodies in stray dogs showed a prevalence of 123 (100%) of the sampled population, whereas in horses, 114 (92.68%) positivity was obtained. Conclusion:The results unveil the prevalence of Influenza A virus in the population of horses and dogs in the state of Nuevo Leon, which could indicate a possible outbreak of equine and Canine Influenza in Mexico. We suggest that the prevalence of Influenza virus in companion animals be monitored to investigate its epizootic and zoonotic potential, in addition to encouraging the regulation of vaccination in these animal species in order to improve their quality of life.

SUBMITTER: Plata-Hipolito CB

PROVIDER: S-EPMC6924343 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Genetic and serologic surveillance of canine (CIV) and equine (EIV) influenza virus in Nuevo León State, México.

Plata-Hipólito Claudia B CB Cedillo-Rosales Sibilina S Obregón-Macías Nelson N Hernández-Luna Carlos E CE Rodríguez-Padilla Cristina C Tamez-Guerra Reyes S RS Contreras-Cordero Juan F JF

PeerJ 20191217

<h4>Background</h4>Despite the uncontrolled distribution of the Influenza A virus through wild birds, the detection of canine influenza virus and equine influenza virus in Mexico was absent until now. Recently, outbreaks of equine and canine influenza have been reported around the world; the virus spreads quickly among animals and there is potential for zoonotic transmission.<h4>Methods</h4>Amplification of the Influenza A virus matrix gene from necropsies, nasal and conjunctival swabs from tras ...[more]

PMID: 31871842

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Project description:BACKGROUND: Trough collections of plants and interviews with 110 individuals, an ethnobotanical study was conducted in order to determine the knowledge and use plant species in Rayones, Nuevo Leon, Mexico. The aim of this study was to record all useful plants and their uses, to know whether differences exist in the knowledge about the number of species and uses between women and men, and to know if there is a correlation between the age of individuals and knowledge of species and their uses. METHODS: A total of 110 persons were interviewed (56 men, 56 women). Semistructured interviews were carried out. The data were analyzed by means of Student t test and the Pearson Correlation Coeficient. RESULTS: A total of 252 species, 228 genera and 91 families of vascular plants were recorded. Astraceae, Fabaceae and are the most important families with useful species and Agave and Opuntia are the genera with the highest number of useful species. One hundred and thirty six species are considered as medicinal. Agave, Acacia and Citrus are the genera with the highest number of medicinal species. Other uses includes edible, spiritual rituals, construction and ornamentals. There was a non-significant correlation between the person's age and number of species, but a significant very low negative correlation between the person's age and number of uses was found. CONCLUSIONS: Knowing their medicinal uses is an important issue for the people of Rayones. Boiling and preparing infusions are the main ways of using plants by residents. The leaves, the branches, and the fruits are the most commonly used parts. Almost 18% of the flora is used for wood and construction purposes. Several uses such as cosmetic, shampoo, firming skin tonics and health hair products recorded in Rayones has not been reported for other areas in the state of Nuevo León. In Rayones, women have a greater knowledge about plants and their uses than men, particularly, medicinal plants, but, men have a greater knowledge about wood and construction species.

Project description:BackgroundAlthough the flora of the State of Nuevo León is well known, there are few records of ethnobotancial information. An ethnobotanical study was undertaken in order to know the medicinal plants used by people living at the scrublands and oak-pine forest areas in the southern Nuevo León. Collection of plants specimens and interviews were carried out among the people of the municipalities of Aramberri, Galeana, and Zaragoza. Since former studies in the region are scarce, the aim of this work was to record the medicinal species and their uses in the scrublands and oak-pine forest areas, of southern Nuevo León, Mexico, and also to know if there are differences in the number of species and number of uses knowledge by people.MethodsField work was carried out over a 2 years period; useful plants were collected and a total of 105 people from 46 different villages were interviewed. A database was compiled using data collected by means of semi structured interviews. The data were analyzed by means of non-parametric statistics, using goodness-of-fit test (Chi-squared) (number of species known by people of each municipality, number of uses known by people of each municipality), Chi-squared modified to incorporate the Yates Correction (number of species known by people living at scrublands and oak-pine forest); the Kruskall-Wallis test (number of species known by women and men of the three municipalities), and the Spearman's rank correlation coefficient (age and number of species known, and age and number of uses).ResultsA total of 163 medicinal plant species were recorded in the study area, comprising 108 wild and 55 cultivated plants. A total of 117 species were recorded in the oak-pine forest, and 111 in the scrublands area, a total of 68 were recorded in both areas; 68 medicinal species are used in all three municipalities, 40 wild and 28 cultivated. We documented 235 different medicinal uses. The most common plant parts used for medicinal purposes were found to be leaves (123 species), stems (55), fruits (28), roots (17), and bark (14). No differences were noted in the number of medicinal plant species identified among people, but differences were significant in their knowledge with respect to the number of uses among people of the three municipalities studied; people from both, scrublands and oak-pine forest know similar number of species and number of uses. Men and women of the three different municipalities knew statistically the same number of species and number of uses. There was no correlation between resident's age and number of species known and resident's age and number of uses either in Galeana or in Aramberri, but, there was high correlation among these variables in Zaragoza.ConclusionIn southern Nuevo León people use at least 5% of the total State flora as medicinal plants, and most of these species are included in few plant families. Most of medicinal species are wild and indigenous to the region. The two most important major plant communities, scrublands and oak-pine forest provide almost the same number of medicinal species. A third of the medicinal flora recorded are used in all three municipalities, most of them are wild. Leaves, stems and fruits are the plant parts most commonly used for healing, and boiling is the most common method used for this purpose. Men and women from the three municipalities are familiar with nearly the same number of species; however, their knowledge of the number of uses varies significantly. In Galeana and Aramberri there was no correlation between a person's age and number of species recognized, however, in Zaragoza, there existed a high correlation between these two factors.

Dataset Information

Genetic and serologic surveillance of canine (CIV) and equine (EIV) influenza virus in Nuevo Leon State, Mexico.

Publications

Genetic and serologic surveillance of canine (CIV) and equine (EIV) influenza virus in Nuevo León State, México.

Similar Datasets

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