Dataset Information

Effect of Sodium Butyrate on LHX1 mRNA Expression as a Transcription Factor of HDAC8 in Human Colorectal Cancer Cell Lines.

ABSTRACT:

Background

LHX1 is an important transcription factor for the HDAC8 gene. The aim of this study was to investigate the effect of Sodium Butyrate (SB), as a histone deacetylase inhibitor, on the expression of LHX1 gene in colorectal cancer cell lines.

Methods

HT-29 and HCT-116 cell lines were treated with 6.25 to 200 mM concentrations of SB at 24, 48, and 72 hr. The cytotoxicity effect on cell viability was evaluated by MTT assay. The 50% Inhibiting Concentration (IC₅₀) was determined graphically. Quantitative real-time PCR was performed to investigate the LHX1 mRNA expression level.

Results

Our study revealed that SB inhibited the proliferation of these cell lines in a concentration and time-dependent manner. The IC₅₀ values for HT-29 cell line were 65, 18.6, and 9.2 mM after 24, 48, and 72 hr of treatment, respectively. The IC₅₀ values for HCT-116 cell line were 35.5, 9.6, and 10 mM after 24, 48, and 72 hr of treatment, respectively. Furthermore, real-time PCR findings demonstrated that the LHX1 mRNA expression in treated HT-29 cell line significantly increased in comparison with untreated cells (p<0.05). However, in treated HCT-116 cell line, SB led to a significant decrease in the level of LHX1 mRNA (p<0.05), as compared to untreated cells.

Conclusion

In this study, different effects of SB on LHX1 mRNA expression level were revealed in two distinct human colorectal cancer cell lines.

SUBMITTER: Ghiaghi M

PROVIDER: S-EPMC6925397 | biostudies-literature | 2019 Oct-Dec

REPOSITORIES: biostudies-literature

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Publications

Effect of Sodium Butyrate on <i>LHX1</i> mRNA Expression as a Transcription Factor of HDAC8 in Human Colorectal Cancer Cell Lines.

Ghiaghi Mahsa M Forouzesh Flora F Rahimi Hamzeh H

Avicenna journal of medical biotechnology 20191001 4

<h4>Background</h4>LHX1 is an important transcription factor for the <i>HDAC8</i> gene. The aim of this study was to investigate the effect of Sodium Butyrate (SB), as a histone deacetylase inhibitor, on the expression of <i>LHX1</i> gene in colorectal cancer cell lines.<h4>Methods</h4>HT-29 and HCT-116 cell lines were treated with 6.25 to 200 <i>mM</i> concentrations of SB at 24, 48, and 72 <i>hr</i>. The cytotoxicity effect on cell viability was evaluated by MTT assay. The 50% Inhibiting Conce ...[more]

PMID: 31908740

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