MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows.
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ABSTRACT:
SUBMITTER: Putz EJ
PROVIDER: S-EPMC6928067 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
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MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows.
Putz Ellie J EJ Putz Austin M AM Jeon Hyeongseon H Lippolis John D JD Ma Hao H Reinhardt Timothy A TA Casas Eduardo E
Scientific reports 20191223 1
In dairy cows, the period from the end of lactation through the dry period and into the transition period, requires vast physiological and immunological changes critical to mammary health. The dry period is important to the success of the next lactation and intramammary infections during the dry period will adversely alter mammary function, health and milk production for the subsequent lactation. MicroRNAs (miRNAs) are small non-coding RNAs that can post transcriptionally regulate gene expressio ...[more]
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2018-01-08 | GSE108840 | GEO
Project description:BackgroundRepeat breeding is a critical reproductive disorder in cattle. The problem of repeat breeder cattle remains largely unmanageable due to a lack of informative biomarkers. Here, we utilized metabolomic profiling in an attempt to identify metabolites in the blood plasma and uterine luminal fluids. We collected blood and uterine fluid from repeat breeder and healthy cows on day 7 of the estrous cycle.ResultsMetabolomic analysis identified 17 plasma metabolites detected at concentrations that distinguished between the two groups, including decreased various bile acids among the repeat breeders. However, no metabolites that varied significantly were detected in the uterine luminal fluids between two groups. Among the plasma samples, kynurenine was identified as undergoing the most significant variation. Kynurenine is a metabolite produced from tryptophan via the actions of indoleamine 2,3-dioxygenase (IDO). As IDO is key for maternal immune tolerance and induced in response to interferon tau (IFNT, ruminant maternal recognition of pregnancy factor), we examined the responsiveness to IFNT on peripheral blood mononuclear cells (PBMC) isolated from healthy and repeat breeder cows. The mRNA expression of IFNT-response makers (ISG15 and MX2) were significantly increased by IFNT treatment in a dose-dependent manner in both groups. Although treatment with IFNT promoted the expression of IDO in PBMCs from both groups, it did so at a substantially reduced rate among the repeat breeder cows, suggesting that decreased levels of kynurenine may relate to the reduced IDO expression in repeat breeder cows.ConclusionsThese findings provide valuable information towards the identification of critical biomarkers for repeat breeding syndrome in cattle.
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