Unknown

Dataset Information

0

Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements.

ABSTRACT: In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and transgenesis of human cells. Further, we demonstrate the versatility of MADR by creating glioma models with mixed reporter-identified zygosity or with "personalized" driver mutations from pediatric glioma. MADR is extensible to thousands of existing mouse lines, providing a flexible platform to democratize the generation of somatic mosaic mice. VIDEO ABSTRACT.

SUBMITTER: Kim GB 

PROVIDER: S-EPMC6934691 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre  ...[more]

Similar Datasets

Transcriptomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis
2019-09-10 | GSE131675 | GEO
Transcriptomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scRNA-seq]
2019-09-10 | GSE117154 | GEO
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scATAC-seq]
2019-09-10 | GSE131873 | GEO
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [ChIP-seq]
2019-09-10 | GSE131672 | GEO
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [bulk ATAC-seq]
2019-09-10 | GSE131940 | GEO
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis
| PRJNA544336 | ENA
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scRNA-seq]
| PRJNA481320 | ENA
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scATAC-seq]
| PRJNA545155 | ENA
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [ChIP-seq]
| PRJNA544338 | ENA
Genomics Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [bulk ATAC-seq]
| PRJNA545352 | ENA