Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scRNA-seq]
Ontology highlight
ABSTRACT: Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scRNA-seq]
Project description:Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis
Project description:Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [ChIP-seq]
Project description:Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [scATAC-seq]
Project description:Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements for studies of gene function in development and tumorigenesis [bulk ATAC-seq]
Project description:In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and transgenesis of human cells. Further, we demonstrate the versatility of MADR by creating glioma models with mixed reporter-identified zygosity or with "personalized" driver mutations from pediatric glioma. MADR is extensible to thousands of existing mouse lines, providing a flexible platform to democratize the generation of somatic mosaic mice. VIDEO ABSTRACT.
Project description:We report the development and efficiency of a new method to create somatic mosaicism in mice named as mosaic analysis by dual recombinase-mediated cassette exchange. Then we generate, as a proof of concept, a new model for pediatric glioma.
Project description:We report the development and eficiency of a new method to create somatic mosaicism in mice named as mosaic analysis by dual recombinase-mediated cassette exchange. Then we generate, as a proof of concept, a new model for pediatric glioima
Project description:We report the development and eficiency of a new method to create somatic mosaicism in mice named as mosaic analysis by dual recombinase-mediated cassette exchange. Then we generate, as a proof of concept, a new model for pediatric glioima
Project description:We report the development and efficiency of a new method to create somatic mosaicism in mice named as mosaic analysis by dual recombinase-mediated cassette exchange. Then we generate, as a proof of concept, a new model for pediatric glioma.