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TagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.

ABSTRACT: Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

SUBMITTER: Nieves DJ

PROVIDER: S-EPMC6936279 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.

Nieves Daniel J DJ Hilzenrat Geva G Tran Jason J Yang Zhengmin Z MacRae Hugh H HH Baker Matthew A B MAB Gooding J Justin JJ Gaus Katharina K

Royal Society open science 20191211 12

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle. ...[more]

PMID: 31903209

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