Ontology highlight
ABSTRACT:
SUBMITTER: Tuley A
PROVIDER: S-EPMC4062584 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Tuley Alfred A Lee Yan-Jiun YJ Wu Bo B Wang Zhiyong U ZU Liu Wenshe R WR
Chemical communications (Cambridge, England) 20140701 56
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling. ...[more]