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Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping.

ABSTRACT: A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of this method to help clinicians in the diagnosis of dyslipidemic and neurodegenerative diseases.

SUBMITTER: Bittar R

PROVIDER: S-EPMC6939034 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping.

Bittar Randa R Carrié Alain A Nouadje Georges G Cherfils Corinne C Fesel-Fouquier Valérie V Barbot-Trystram Laurence L Giral Philippe P Bonnefont-Rousselot Dominique D

Practical laboratory medicine 20191217

A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of ...[more]

PMID: 31909147

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