Dataset Information

High-Throughput Targeted Quantitative Analysis of the Interaction between HSP90 and Kinases.

ABSTRACT: Kinases, which function in numerous cell signaling processes, are among the best characterized groups of client proteins for the 90-kDa heat shock protein (HSP90), a molecular chaperone that suppresses the aggregation and maintains the proper folding of its substrate proteins (i.e., clients). No high-throughput proteomic method, however, has been developed for the characterizations of the interactions between HSP90 and the human kinome. Herein, by employing a parallel-reaction monitoring (PRM)-based targeted proteomic method, we found that 99 out of the 249 detected kinase proteins display diminished expression in cultured human cells upon treatment with ganetespib, a small-molecule HSP90 inhibitor. PRM analysis of kinase proteins in the affinity pull-down samples showed that 86 out of the 120 detected kinases are enriched from the CRISPR-engineered cells where a tandem affinity tag was conjugated with the C-terminus of endogenous HSP90? protein over the parental cells. Together, our results from the two complementary quantitative proteomic experiments offer systematic characterizations about the HSP90-kinase interactions at the entire proteome scale and reveal extensive interactions between HSP90 and kinase proteins in human cells.

SUBMITTER: Miao W

PROVIDER: S-EPMC6939611 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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High-Throughput Targeted Quantitative Analysis of the Interaction between HSP90 and Kinases.

Miao Weili W Li Lin L Wang Yinsheng Y

Analytical chemistry 20190904 18

Kinases, which function in numerous cell signaling processes, are among the best characterized groups of client proteins for the 90-kDa heat shock protein (HSP90), a molecular chaperone that suppresses the aggregation and maintains the proper folding of its substrate proteins (i.e., clients). No high-throughput proteomic method, however, has been developed for the characterizations of the interactions between HSP90 and the human kinome. Herein, by employing a parallel-reaction monitoring (PRM)-b ...[more]

PMID: 31476117

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Dataset Information

High-Throughput Targeted Quantitative Analysis of the Interaction between HSP90 and Kinases.

Publications

High-Throughput Targeted Quantitative Analysis of the Interaction between HSP90 and Kinases.

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