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Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1.


ABSTRACT: Duck astrovirus type 1 (DAstV-1) infection constitutes a cause of viral hepatitis in ducklings and little is known about the B-cell epitope of DAstV-1. In this study, a monoclonal antibody (mAb) 3D2 against open reading frame 2 (ORF2) protein of DAstV-1 was used to identify the possible epitope in the four serotypes of DAstV. The mAb 3D2 showed no neutralization activity to DAstV-1, and reacted with the conserved linear B-cell epitopes of 454STTESA459 in DAstV-1 ORF2 protein. Sequence analysis, dot blot assay, and cross-reactivity test indicated that the epitope peptide was highly conserved in DAstV-1 sequence and mAb 3D2 had no cross-reactivity with other DAstV serotypes. To the best of our knowledge, this is the first report about identification of the specific conserved linear B-cell epitope of DAstV-1, which will facilitate the serologic diagnosis of DAstV-1 infection.

SUBMITTER: Lan J 

PROVIDER: S-EPMC6940979 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1.

Lan Jingjing J   Zhang Ruihua R   Li Pengfei P   Chen Junhao J   Xie Zhijing Z   Jiang Shijin S  

Animals : an open access journal from MDPI 20191202 12


Duck astrovirus type 1 (DAstV-1) infection constitutes a cause of viral hepatitis in ducklings and little is known about the B-cell epitope of DAstV-1. In this study, a monoclonal antibody (mAb) 3D2 against open reading frame 2 (ORF2) protein of DAstV-1 was used to identify the possible epitope in the four serotypes of DAstV. The mAb 3D2 showed no neutralization activity to DAstV-1, and reacted with the conserved linear B-cell epitopes of <sup>454</sup>STTESA<sup>459</sup> in DAstV-1 ORF2 protei  ...[more]

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