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Conditional control of RNA-guided nucleic acid cleavage and gene editing.

ABSTRACT: Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.

SUBMITTER: Wang SR

PROVIDER: S-EPMC6941951 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Conditional control of RNA-guided nucleic acid cleavage and gene editing.

Wang Shao-Ru SR Wu Ling-Yu LY Huang Hai-Yan HY Xiong Wei W Liu Jian J Wei Lai L Yin Ping P Tian Tian T Zhou Xiang X

Nature communications 20200103 1

Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure pr ...[more]

PMID: 31900392

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