Ontology highlight
ABSTRACT:
SUBMITTER: Wang SR
PROVIDER: S-EPMC6941951 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Wang Shao-Ru SR Wu Ling-Yu LY Huang Hai-Yan HY Xiong Wei W Liu Jian J Wei Lai L Yin Ping P Tian Tian T Zhou Xiang X
Nature communications 20200103 1
Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure pr ...[more]