Ontology highlight
ABSTRACT:
SUBMITTER: Villette V
PROVIDER: S-EPMC6941988 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Villette Vincent V Chavarha Mariya M Dimov Ivan K IK Bradley Jonathan J Pradhan Lagnajeet L Mathieu Benjamin B Evans Stephen W SW Chamberland Simon S Shi Dongqing D Yang Renzhi R Kim Benjamin B BB Ayon Annick A Jalil Abdelali A St-Pierre François F Schnitzer Mark J MJ Bi Guoqiang G Toth Katalin K Ding Jun J Dieudonné Stéphane S Lin Michael Z MZ
Cell 20191201 7
Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with ...[more]