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Upregulation of p53 through induction of MDM2 degradation: Amino acid prodrugs of anthraquinone analogs.

ABSTRACT: Previously, we reported a class of MDM2-MDM4 dimerization inhibitors that upregulate p53 and showed potent anticancer activity in animal models. However, water solubility hinders their further development. Herein we describe our effort to develop a prodrug approach that overcomes the solubility problem. The prodrug of BW-AQ-238, a potent anthraquinone analog, was made by esterification of the hydroxyl group with various natural amino acids. Cytotoxicity of these compounds toward Hela and EU-1 cells, their aqueous solubility, and the release kinetics of these prodrugs in buffer and in the presence of hydrolytic enzymes were studied. The results demonstrate that the amino acid prodrug approach significantly improved the water solubility while maintaining the potency of the parent drug.

SUBMITTER: Anifowose A

PROVIDER: S-EPMC6942214 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Upregulation of p53 through induction of MDM2 degradation: Amino acid prodrugs of anthraquinone analogs.

Anifowose Abiodun A Yuan Zhengnan Z Yang Xiaoxiao X Pan Zhixiang Z Zheng Yueqin Y Zhang Zhongwei Z Wang Binghe B

Bioorganic & medicinal chemistry letters 20191111 2

Previously, we reported a class of MDM2-MDM4 dimerization inhibitors that upregulate p53 and showed potent anticancer activity in animal models. However, water solubility hinders their further development. Herein we describe our effort to develop a prodrug approach that overcomes the solubility problem. The prodrug of BW-AQ-238, a potent anthraquinone analog, was made by esterification of the hydroxyl group with various natural amino acids. Cytotoxicity of these compounds toward Hela and EU-1 ce ...[more]

PMID: 31753697

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