Dataset Information

A Microfluidic Rotational Motor Driven by Circular Vibrations.

ABSTRACT: Constructing micro-sized machines always involves the problem of how to bring the energy (electric, magnetic, light, electro wetting, vibrational, etc.) source to the device to produce mechanical movements. The paper presents a rotational micro-sized motor (the diameter of the rotor is 350 µm) driven by low frequency (200-700 Hz) circular vibrations, made by two piezoelectric actuators, through the medium of a water droplet with diameter of 1 mm (volume 3.6 µL). The theoretical model presents how to produce the circular streaming (rotation) of the liquid around an infinitely long pillar with micro-sized diameter. The practical application has been focused to make a time-stable circular stream of the medium around the finite long vibrated pillar with diameter of 80 µm in the presence of disturbances produced by the vibrated plate where the pillar is placed. Only the time-stable circular stream in the water droplet around the pillar produces enough energy to rotate the micro-sized rotor. The rotational speed of the rotor is controlled in both directions from -20 rad/s to +26 rad/s. 3D printed mechanical amplifiers of vibrations, driven by piezoelectric actuators, amplify the amplitude of the piezoelectric actuator up to 20 µm in the frequency region of 200 to 700 Hz.

SUBMITTER: Uran S

PROVIDER: S-EPMC6953025 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A Microfluidic Rotational Motor Driven by Circular Vibrations.

Uran Suzana S Bratina Božidar B Šafarič Riko R

Micromachines 20191123 12

Constructing micro-sized machines always involves the problem of how to bring the energy (electric, magnetic, light, electro wetting, vibrational, etc.) source to the device to produce mechanical movements. The paper presents a rotational micro-sized motor (the diameter of the rotor is 350 µm) driven by low frequency (200-700 Hz) circular vibrations, made by two piezoelectric actuators, through the medium of a water droplet with diameter of 1 mm (volume 3.6 µL). The theoretical model presents ho ...[more]

PMID: 31771192

Similar Datasets

Project description:The bacterial flagellar motor can rotate either clockwise (CW) or counterclockwise (CCW). Three flagellar proteins, FliG, FliM, and FliN, are required for rapid switching between the CW and CCW directions. Switching is achieved by a conformational change in FliG induced by the binding of a chemotaxis signaling protein, phospho-CheY, to FliM and FliN. FliG consists of three domains, FliG(N), FliG(M), and FliG(C), and forms a ring on the cytoplasmic face of the MS ring of the flagellar basal body. Crystal structures have been reported for the FliG(MC) domains of Thermotoga maritima, which consist of the FliG(M) and FliG(C) domains and a helix E that connects these two domains, and full-length FliG of Aquifex aeolicus. However, the basis for the switching mechanism is based only on previously obtained genetic data and is hence rather indirect. We characterized a CW-biased mutant (fliG(?PAA)) of Salmonella enterica by direct observation of rotation of a single motor at high temporal and spatial resolution. We also determined the crystal structure of the FliG(MC) domains of an equivalent deletion mutant variant of T. maritima (fliG(?PEV)). The FliG(?PAA) motor produced torque at wild-type levels under a wide range of external load conditions. The wild-type motors rotated exclusively in the CCW direction under our experimental conditions, whereas the mutant motors rotated only in the CW direction. This result suggests that wild-type FliG is more stable in the CCW state than in the CW state, whereas FliG(?PAA) is more stable in the CW state than in the CCW state. The structure of the TM-FliG(MC)(?PEV) revealed that extremely CW-biased rotation was caused by a conformational change in helix E. Although the arrangement of FliG(C) relative to FliG(M) in a single molecule was different among the three crystals, a conserved FliG(M)-FliG(C) unit was observed in all three of them. We suggest that the conserved FliG(M)-FliG(C) unit is the basic functional element in the rotor ring and that the PAA deletion induces a conformational change in a hinge-loop between FliG(M) and helix E to achieve the CW state of the FliG ring. We also propose a novel model for the arrangement of FliG subunits within the motor. The model is in agreement with the previous mutational and cross-linking experiments and explains the cooperative switching mechanism of the flagellar motor.

Dataset Information

A Microfluidic Rotational Motor Driven by Circular Vibrations.

Publications

A Microfluidic Rotational Motor Driven by Circular Vibrations.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets