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A Pot-Like Vibrational Microfluidic Rotational Motor.

ABSTRACT: Constructing a micro-sized microfluidic motor always involves the problem of how to transfer the mechanical energy out of the motor. The paper presents several experiments with pot-like microfluidic rotational motor structures driven by two perpendicular sine and cosine vibrations with amplitudes around 10 ?m in the frequency region from 200 Hz to 500 Hz. The extensive theoretical research based on the mathematical model of the liquid streaming in a pot-like structure was the base for the successful real-life laboratory application of a microfluidic rotational motor. The final microfluidic motor structure allowed transferring the rotational mechanical energy out of the motor with a central axis. The main practical challenge of the research was to find the proper balance between the torque, due to friction in the bearings and the motor's maximal torque. The presented motor, with sizes 1 mm by 0.6 mm, reached the maximal rotational speed in both directions between -15 rad/s to +14 rad/s, with the estimated maximal torque of 0.1 pNm. The measured frequency characteristics of vibration amplitudes and phase angle between the directions of both vibrational amplitudes and rotational speed of the motor rotor against frequency of vibrations, allowed us to understand how to build the pot-like microfluidic rotational motor.

SUBMITTER: Uran S

PROVIDER: S-EPMC7918734 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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A Pot-Like Vibrational Microfluidic Rotational Motor.

Uran Suzana S Malok Matjaž M Bratina Božidar B Šafarič Riko R

Micromachines 20210211 2

Constructing a micro-sized microfluidic motor always involves the problem of how to transfer the mechanical energy out of the motor. The paper presents several experiments with pot-like microfluidic rotational motor structures driven by two perpendicular sine and cosine vibrations with amplitudes around 10 μm in the frequency region from 200 Hz to 500 Hz. The extensive theoretical research based on the mathematical model of the liquid streaming in a pot-like structure was the base for the succes ...[more]

PMID: 33670259

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Project description:The bacterial flagellar motor can rotate either clockwise (CW) or counterclockwise (CCW). Three flagellar proteins, FliG, FliM, and FliN, are required for rapid switching between the CW and CCW directions. Switching is achieved by a conformational change in FliG induced by the binding of a chemotaxis signaling protein, phospho-CheY, to FliM and FliN. FliG consists of three domains, FliG(N), FliG(M), and FliG(C), and forms a ring on the cytoplasmic face of the MS ring of the flagellar basal body. Crystal structures have been reported for the FliG(MC) domains of Thermotoga maritima, which consist of the FliG(M) and FliG(C) domains and a helix E that connects these two domains, and full-length FliG of Aquifex aeolicus. However, the basis for the switching mechanism is based only on previously obtained genetic data and is hence rather indirect. We characterized a CW-biased mutant (fliG(?PAA)) of Salmonella enterica by direct observation of rotation of a single motor at high temporal and spatial resolution. We also determined the crystal structure of the FliG(MC) domains of an equivalent deletion mutant variant of T. maritima (fliG(?PEV)). The FliG(?PAA) motor produced torque at wild-type levels under a wide range of external load conditions. The wild-type motors rotated exclusively in the CCW direction under our experimental conditions, whereas the mutant motors rotated only in the CW direction. This result suggests that wild-type FliG is more stable in the CCW state than in the CW state, whereas FliG(?PAA) is more stable in the CW state than in the CCW state. The structure of the TM-FliG(MC)(?PEV) revealed that extremely CW-biased rotation was caused by a conformational change in helix E. Although the arrangement of FliG(C) relative to FliG(M) in a single molecule was different among the three crystals, a conserved FliG(M)-FliG(C) unit was observed in all three of them. We suggest that the conserved FliG(M)-FliG(C) unit is the basic functional element in the rotor ring and that the PAA deletion induces a conformational change in a hinge-loop between FliG(M) and helix E to achieve the CW state of the FliG ring. We also propose a novel model for the arrangement of FliG subunits within the motor. The model is in agreement with the previous mutational and cross-linking experiments and explains the cooperative switching mechanism of the flagellar motor.

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A Pot-Like Vibrational Microfluidic Rotational Motor.

Publications

A Pot-Like Vibrational Microfluidic Rotational Motor.

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