Dataset Information

Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB.

ABSTRACT: Focal adhesion kinase (FAK) is essential for vascular endothelial growth factor-A (VEGFA)/VEGF receptor-2 (VEGFR2)-stimulated angiogenesis and vascular permeability. We have previously noted that presence of the Src homology-2 domain adapter protein B (SHB) is of relevance for VEGFA-stimulated angiogenesis in a FAK-dependent manner. The current study was conducted in order address the temporal dynamics of co-localization between these components in HEK293 and primary lung endothelial cells (EC) by total internal reflection fluorescence microscopy (TIRF). An early (<2.5 min) VEGFA-induced increase in VEGFR2 co-localization with SHB was dependent on tyrosine 1175 in VEGFR2. VEGFA also enhanced SHB co-localization with FAK. FAK co-localization with VEGFR2 was dependent on SHB since it was significantly lower in SHB deficient EC after VEGFA addition. Absence of SHB also resulted in a gradual decline of VEGFR2 co-localization with FAK under basal (prior to VEGFA addition) conditions. A similar basal response was observed with expression of the Y1175F-VEGFR2 mutant in wild type EC. The distribution of focal adhesions in SHB-deficient EC was altered with a primarily perinuclear location. These live cell data implicate SHB as a key component regulating FAK activity in response to VEGFA/VEGFR2.

SUBMITTER: Pietila I

PROVIDER: S-EPMC6953046 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB.

Pietilä Ilkka I Van Mourik Djenolan D Tamelander Andreas A Kriz Vitezslav V Claesson-Welsh Lena L Tengholm Anders A Welsh Michael M

Cells 20191215 12

Focal adhesion kinase (FAK) is essential for vascular endothelial growth factor-A (VEGFA)/VEGF receptor-2 (VEGFR2)-stimulated angiogenesis and vascular permeability. We have previously noted that presence of the Src homology-2 domain adapter protein B (SHB) is of relevance for VEGFA-stimulated angiogenesis in a FAK-dependent manner. The current study was conducted in order address the temporal dynamics of co-localization between these components in HEK293 and primary lung endothelial cells (EC) ...[more]

PMID: 31847469

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Dataset Information

Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB.

Publications

Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB.

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