Alterations of aqueous humor A? levels in A?-infused and transgenic mouse models of Alzheimer disease.

ABSTRACT: Alzheimer's disease (AD) is an ageing-related neurodegenerative disease characterized and diagnosed by deposition of insoluble amyloid-? (A?) plaques in the brain. The plaque accumulation in the brain directly affects reduced levels of A? in cerebrospinal fluid (CSF) and blood, as A? can freely transport the blood-brain barrier, and clinical investigations have suggested these two biofluids as promising samples for in vitro diagnosis. Given that the human eye structurally resembles the brain and A? accumulation often observed in the ocular region of AD patients, in this study, we examined aqueous humor A? as another possible surrogate biomarker. First, using the acute A?-infused AD mouse model by injecting A? to the CSF in intracerebroventricular region of normal ICR mice, we investigated whether A? concentration in the aqueous humor in AD models is positively correlated with the concentration in the CSF. Then, we examined the correlation of aqueous humor A? levels with increased plaque deposition in the brain and reduced A? levels in both CSF and blood in adult and aged 5XFAD Alzheimer transgenic mice. Collectively, the synthetic A? injected into CSF immediately migrate to the aqueous humor, however, the age-dependently reducing pattern of A? levels in CSF and blood was not observed in the aqueous humor.

SUBMITTER: Kwak DE 

PROVIDER: S-EPMC6953883 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature