Lung-protective ventilation worsens ventilator-induced diaphragm atrophy and weakness.

ABSTRACT: BACKGROUND:Lung-protective ventilation (LPV) has been found to minimize the risk of ventilator-induced lung injury (VILI). However, whether LPV is able to diminish ventilator-induced diaphragm dysfunction (VIDD) remains unknown. This study was designed to test the hypothesis that LPV protects the diaphragm against VIDD. METHODS:Adult male Wistar rats received either conventional mechanical (tidal volume [VT]: 10?ml/kg, positive end-expiratory pressure [PEEP]: 2?cm H2O; CV group) or lung-protective (VT: 5?ml/kg, PEEP: 10?cm H2O; LPV group) ventilation for 12?h. Then, diaphragms and lungs were collected for biochemical and histological analyses. Transcriptome sequencing (RNA-seq) was performed to determine the differentially expressed genes in the diaphragms between groups. RESULTS:Our results suggested that LPV was associated with diminished pulmonary injuries and reduced oxidative stress compared with the effects of the CV strategy in rats. However, animals that received LPV showed increased protein degradation, decreased cross-sectional areas (CSAs) of myofibers, and reduced forces of the diaphragm compared with the same parameters in animals receiving CV (p?

SUBMITTER: Zhou XL 

PROVIDER: S-EPMC6954632 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature