Unknown

Dataset Information

0

The adaptor proteins HAP1a and GRIP1 collaborate to activate the kinesin-1 isoform KIF5C.


ABSTRACT: Binding of motor proteins to cellular cargoes is regulated by adaptor proteins. HAP1 and GRIP1 are kinesin-1 adaptors that have been implicated individually in the transport of vesicular cargoes in the dendrites of neurons. We find that HAP1a and GRIP1 form a protein complex in the brain, and co-operate to activate the kinesin-1 subunit KIF5C in vitro Based upon this co-operative activation of kinesin-1, we propose a modification to the kinesin activation model that incorporates stabilisation of the central hinge region known to be critical to autoinhibition of kinesin-1.

SUBMITTER: Twelvetrees AE 

PROVIDER: S-EPMC6955223 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

The adaptor proteins HAP1a and GRIP1 collaborate to activate the kinesin-1 isoform KIF5C.

Twelvetrees Alison E AE   Lesept Flavie F   Holzbaur Erika L F ELF   Kittler Josef T JT  

Journal of cell science 20191213 24


Binding of motor proteins to cellular cargoes is regulated by adaptor proteins. HAP1 and GRIP1 are kinesin-1 adaptors that have been implicated individually in the transport of vesicular cargoes in the dendrites of neurons. We find that HAP1a and GRIP1 form a protein complex in the brain, and co-operate to activate the kinesin-1 subunit KIF5C <i>in vitro</i> Based upon this co-operative activation of kinesin-1, we propose a modification to the kinesin activation model that incorporates stabilisa  ...[more]

Similar Datasets

| S-EPMC7799768 | biostudies-literature
| S-EPMC9896810 | biostudies-literature
| EMPIAR-11357 | biostudies-other
| S-EPMC7226124 | biostudies-literature
| S-EPMC3400561 | biostudies-literature
| S-EPMC7305210 | biostudies-literature
| S-EPMC3599065 | biostudies-literature
| S-EPMC8319186 | biostudies-literature
| S-EPMC2063617 | biostudies-literature
| S-EPMC2268436 | biostudies-literature