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Physiological and Proteomic Approaches to Address the Active Role of Botrytis cinerea Inoculation in Tomato Postharvest Ripening.

ABSTRACT: Botrytis cinerea is an unbearable postharvest threat with significant economic impacts. Necrotrophic B. cinerea can readily infect ripe fruit resulting in the rapid progression of symptoms of the disease. To unravel the mechanism by which tomato fruit opposes pathogen attack, we investigated the changes in quality-related attributes as a direct response (DR) or systemic response (SR) of infected tomatoes to the B. cinerea. Additionally, the SR of protein yield and composition were studied in fruit stored at 11 °C/90% relative humidity (RH) for one week. Fungal infection accelerated ripening with increased ethylene and respiration rates. Fruit softening, ascorbic acid and ?-carotene increase were associated with DR but not with the SR of the pathogen. Pathogen infection increased lipid peroxidation, causing the production of hydrogen peroxide and oxidative stress, as fruit activated both enzymatic and non-enzymatic mechanisms to trigger stress. B. cinerea increased up to 6.6% the protein yield and downregulated at least 39 proteins. Proteins involved in fruit ripening, such as an ethylene biosynthetic enzyme, were increased in wound-inoculated fruit. Moreover, antioxidant proteins, such as ascorbate peroxidase-APX1 and superoxide dismutase-SOD, increased in infected tomatoes, as these proteins are involved in reactive oxygen species detoxification. Constitutively-expressed proteins tended to be either increased (chaperonin and malate dehydrogenase) or remained unaffected (dehydrin) by pathogen inoculation. Protein levels involved in the metabolism of carbohydrate, the pentose phosphate pathway, terpenoid and flavonoid biosynthesis were differently affected during the treatments. By enabling a better understanding of the fungal direct or systemic response on fruit quality and ripening through biochemical and proteome studies, we may improve the plant-pathogen interaction and complexity.

SUBMITTER: Tzortzakis N

PROVIDER: S-EPMC6955909 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Physiological and Proteomic Approaches to Address the Active Role of Botrytis cinerea Inoculation in Tomato Postharvest Ripening.

Tzortzakis Nikolaos N

Microorganisms 20191211 12

Botrytis cinerea is an unbearable postharvest threat with significant economic impacts. Necrotrophic B. cinerea can readily infect ripe fruit resulting in the rapid progression of symptoms of the disease. To unravel the mechanism by which tomato fruit opposes pathogen attack, we investigated the changes in quality-related attributes as a direct response (DR) or systemic response (SR) of infected tomatoes to the B. cinerea. Additionally, the SR of protein yield and compositio ...[more]

PMID: 31835786

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Project description:Worldwide, 20-25% of all harvested fruit and vegetables are lost annually in the field and throughout the postharvest supply chain due to rotting by fungal pathogens. Most postharvest pathogens exhibit necrotrophic or saprotrophic lifestyles, resulting in decomposition of the host tissues and loss of marketable commodities. Necrotrophic fungi can readily infect ripe fruit leading to the rapid establishment of disease symptoms. However, these pathogens generally fail to infect unripe fruit or remain quiescent until host conditions stimulate a successful infection. Previous research on infections of fruit has mainly been focused on the host's genetic and physicochemical factors that inhibit or promote disease. Here, we investigated if fruit pathogens can modify their own infection strategies in response to the ripening stage of the host. To test this hypothesis, we profiled global gene expression of three fungal pathogens that display necrotrophic behavior-Botrytis cinerea, Fusarium acuminatum, and Rhizopus stolonifer-during interactions with unripe and ripe tomato fruit. We assembled and functionally annotated the transcriptomes of F. acuminatum and R. stolonifer as no genomic resources were available. Then, we conducted differential gene expression analysis to compare each pathogen during inoculations versus in vitro conditions. Through characterizing patterns of overrepresented pathogenicity and virulence functions (e.g., phytotoxin production, cell wall degradation, and proteolysis) among the differentially expressed genes, we were able to determine shared strategies among the three fungi during infections of compatible (ripe) and incompatible (unripe) fruit tissues. Though each pathogen's strategy differed in the details, interactions with unripe fruit were commonly characterized by an emphasis on the degradation of cell wall components, particularly pectin, while colonization of ripe fruit featured more heavily redox processes, proteolysis, metabolism of simple sugars, and chitin biosynthesis. Furthermore, we determined that the three fungi were unable to infect fruit from the non-ripening (nor) tomato mutant, confirming that to cause disease, these pathogens require the host tissues to undergo specific ripening processes. By enabling a better understanding of fungal necrotrophic infection strategies, we move closer to generating accurate models of fruit diseases and the development of early detection tools and effective management strategies.

Dataset Information

Physiological and Proteomic Approaches to Address the Active Role of Botrytis cinerea Inoculation in Tomato Postharvest Ripening.

Publications

Physiological and Proteomic Approaches to Address the Active Role of <i>Botrytis cinerea</i> Inoculation in Tomato Postharvest Ripening.

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