Ontology highlight
ABSTRACT:
SUBMITTER: Wang Z
PROVIDER: S-EPMC6961706 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Wang Zijun Z Merkenschlager Julia J Chen Spencer T ST Oliveira Thiago Y TY Ramos Victor V Gordon Kristie M KM Yao Kai-Hui KH Jankovic Mila M Nussenzweig Michel M Escolano Amelia A
Journal of immunological methods 20191219
Antibody cloning from single B cells is an essential tool for characterizing humoral immune responses and obtaining valuable therapeutic and analytical reagents. Antibody cloning from individuals with high serologic titers to HIV-1, Influenza, Malaria and ZIKV has led to new insights that inform vaccine design efforts. In contrast to humans and mice, less is known about antibody cloning from single B cells in macaques. Here, we describe a protocol to identify and purify single antigen-specific m ...[more]