Dataset Information

Polycaprolactone-Based, Porous CaCO3 and Ag Nanoparticle Modified Scaffolds as a SERS Platform With Molecule-Specific Adsorption.

ABSTRACT: Surface-enhanced Raman scattering (SERS) is a high-performance technique allowing detection of extremely low concentrations of analytes. For such applications, fibrous polymeric matrices decorated with plasmonic metal nanostructures can be used as flexible SERS substrates for analysis of analytes in many application. In this study, a three-dimensional SERS substrate consisting of a CaCO3-mineralized electrospun (ES) polycaprolactone (PCL) fibrous matrix decorated with silver (Ag) nanoparticles is developed. Such modification of the fibrous substrate allows achieving a significant increase of the SERS signal amplification. Functionalization of fibers by porous CaCO3 (vaterite) and Ag nanoparticles provides an effective approach of selective adsorption of biomolecules and their precise detection by SERS. This new SERS substrate represents a promising biosensor platform with selectivity to low and high molecular weight molecules.

SUBMITTER: Saveleva M

PROVIDER: S-EPMC6967418 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Polycaprolactone-Based, Porous CaCO<sub>3</sub> and Ag Nanoparticle Modified Scaffolds as a SERS Platform With Molecule-Specific Adsorption.

Saveleva Mariia M Prikhozhdenko Ekaterina E Gorin Dmitry D Skirtach Andre G AG Yashchenok Alexey A Parakhonskiy Bogdan B

Frontiers in chemistry 20200110

Surface-enhanced Raman scattering (SERS) is a high-performance technique allowing detection of extremely low concentrations of analytes. For such applications, fibrous polymeric matrices decorated with plasmonic metal nanostructures can be used as flexible SERS substrates for analysis of analytes in many application. In this study, a three-dimensional SERS substrate consisting of a CaCO<sub>3</sub>-mineralized electrospun (ES) polycaprolactone (PCL) fibrous matrix decorated with silver (Ag) nano ...[more]

PMID: 31998685

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Project description:Biomaterial-associated infections are one of the major causes of implant failure. These infections result from persistent bacteria that have adhered to the biomaterial surface before, during, or after surgery and have formed a biofilm on the implant's surface. It is estimated that 4 to 10% of implant surfaces are contaminated with bacteria; however, the infection rate can be as high as 30% in intensive care units in developed countries and as high as 45% in developing countries. To date, there is no clinical solution to prevent implant infection without relying on the use of high doses of antibiotics supplied systemically and/or removal of the infected device. In this study, melimine, a chimeric cationic peptide that has been tested in Phase I and II human clinical trials, was immobilized onto the surface of 3D-printed medical-grade polycaprolactone (mPCL) scaffolds via covalent binding and adsorption. X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) spectra of melimine-treated surfaces confirmed immobilization of the peptide, as well as its homogeneous distribution throughout the scaffold surface. Amino acid analysis showed that melimine covalent and noncovalent immobilization resulted in a peptide density of ∼156 and ∼533 ng/cm2, respectively. Furthermore, we demonstrated that the immobilization of melimine on mPCL scaffolds by 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) coupling and noncovalent interactions resulted in a reduction of Staphylococcus aureus colonization by 78.7% and 76.0%, respectively, in comparison with the nonmodified control specimens. Particularly, the modified surfaces maintained their antibacterial properties for 3 days, which resulted in the inhibition of biofilm formation in vitro. This system offers a biomaterial strategy to effectively prevent biofilm-related infections on implant surfaces without relying on the use of prophylactic antibiotic treatment.

Dataset Information

Polycaprolactone-Based, Porous CaCO3 and Ag Nanoparticle Modified Scaffolds as a SERS Platform With Molecule-Specific Adsorption.

Publications

Polycaprolactone-Based, Porous CaCO<sub>3</sub> and Ag Nanoparticle Modified Scaffolds as a SERS Platform With Molecule-Specific Adsorption.

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