MicroRNA-7, synergizes with ROR?, negatively controls the pathology of brain tissue inflammation.

ABSTRACT: BACKGROUND:Accumulating evidence has documented that microRNA-7 (miR-7) plays an important role in the pathology of various diseases. However, the potential role of miR-7 in brain tissue inflammation (BTI) remains unclear. METHODS:We detected the expression of miR-7 in LPS-induced murine BTI model and observed the possible effects of miR-7 deficiency on the pathology of BTI. To elucidate the mechanism, the target gene of miR-7 was screened out by Gene chip assay and its potential roles in BTI were evaluated by Western blot, immunofluorescence, and RNAi assay, respectively. RESULTS:MiR-7 was upregulated in brain tissue in BTI mice and its deficiency could significantly aggravate the pathology of brain tissue. Moreover, ROR?, a new target molecule of miR-7, was upregulated in brain tissue from miR-7 deficiency BTI mice. Of note, downregulation of ROR? could remarkably exacerbate the pathology of brain tissue and elevate the transduction of NF-?B and ERK1/2 signaling pathways in brain tissue from miR-7 deficiency BTI mice. Furthermore, ROR? and miR-7 were dominantly co-expressed in neurons of BTI mice. Finally, ROR? synergized with miR-7 to control the inflammatory reaction of neuronal cells in response to LPS stimulation. CONCLUSIONS:MiR-7 expression is upregulated in BTI model. Moreover, miR-7 synergizes with its target gene ROR? to control the inflammation reaction of neurons, thereby orchestrating the pathology of BTI.

SUBMITTER: Yue D 

PROVIDER: S-EPMC6970296 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature