Dataset Information

Distribution of Bacterial ?1,3-Galactosyltransferase Genes in the Human Gut Microbiome.

ABSTRACT: Because of a loss-of-function mutation in the GGTA1 gene, humans are unable to synthetize ?1,3-Galactose (Gal) decorated glycans and develop high levels of circulating anti-?1,3-Galactose antibodies (anti-Gal Abs). Anti-Gal Abs have been identified as a major obstacle of organ xenotransplantation and play a role in several host-pathogen relationships including potential susceptibility to infection. Anti-Gal Abs are supposed to stem from immunization against the gut microbiota, an assumption derived from the observation that some pathogens display ?1,3-Gal and that antibiotic treatment decreases the level of anti-Gal. However, there is little information to date concerning the microorganisms producing ?1,3-Gal in the human gut microbiome. Here, available ?1,3-Galactosyltransferase (GT) gene sequences from gut bacteria were selectively quantified for the first time in the gut microbiome shotgun sequences of 163 adult individuals from three published population-based metagenomics analyses. We showed that most of the gut microbiome of adult individuals contained a small set of bacteria bearing ?1,3-GT genes. These bacteria belong mainly to the Enterobacteriaceae family, including Escherichia coli, but also to Pasteurellaceae genera, Haemophilus influenza and Lactobacillus species. ?1,3-Gal antigens and ?1,3-GT activity were detected in healthy stools of individuals exhibiting ?1,3-GT bacterial gene sequences in their shotgun data.

SUBMITTER: Montassier E

PROVIDER: S-EPMC6970434 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome.

Montassier Emmanuel E Al-Ghalith Gabriel A GA Mathé Camille C Le Bastard Quentin Q Douillard Venceslas V Garnier Abel A Guimon Rémi R Raimondeau Bastien B Touchefeu Yann Y Duchalais Emilie E Vince Nicolas N Limou Sophie S Gourraud Pierre-Antoine PA Laplaud David A DA Nicot Arnaud B AB Soulillou Jean-Paul JP Berthelot Laureline L

Frontiers in immunology 20200113

Because of a loss-of-function mutation in the GGTA1 gene, humans are unable to synthetize α1,3-Galactose (Gal) decorated glycans and develop high levels of circulating anti-α1,3-Galactose antibodies (anti-Gal Abs). Anti-Gal Abs have been identified as a major obstacle of organ xenotransplantation and play a role in several host-pathogen relationships including potential susceptibility to infection. Anti-Gal Abs are supposed to stem from immunization against the gut microbiota, an assumption deri ...[more]

PMID: 31998300

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Dataset Information

Distribution of Bacterial ?1,3-Galactosyltransferase Genes in the Human Gut Microbiome.

Publications

Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome.

Similar Datasets

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