Dataset Information

RNA sequencing data of tobacco inoculated with Apple stem pitting virus.

ABSTRACT: Apple stem pitting virus (ASPV) mainly infects apple, pear and their closely related species in the world. ASPV causes some symptoms like leaf etiolation and stony pit in cultivated pear, but produces few symptoms in cultivated apple. We inoculated tobacco with ASPV, which originates from Nanking cherry (Prunus tomentosa), and we obtained tobacco RNA-sequencing data through high-throughput sequencing. In total, 17,401,736 clean reads were obtained after sequencing and removing adaptor sequences, contamination and low-quality reads. An RNA-seq data has been uploaded to Sequence Read Archive (SRA), which enables researchers to access the RNA-sequencing data of tobacco inoculated with ASPV.

SUBMITTER: Yan G

PROVIDER: S-EPMC6971375 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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RNA sequencing data of tobacco inoculated with Apple stem pitting virus.

Yan Gengxuan G Yang Hongyi H

Data in brief 20200103

Apple stem pitting virus (ASPV) mainly infects apple, pear and their closely related species in the world. ASPV causes some symptoms like leaf etiolation and stony pit in cultivated pear, but produces few symptoms in cultivated apple. We inoculated tobacco with ASPV, which originates from Nanking cherry (Prunus tomentosa), and we obtained tobacco RNA-sequencing data through high-throughput sequencing. In total, 17,401,736 clean reads were obtained after sequencing and removing adap ...[more]

PMID: 31993465

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Project description:BACKGROUND:Although the canonical function of viral coat protein (CP) is to encapsidate the viral genome, they have come to be recognized as multifunctional proteins, involved in almost every stage of the viral infection cycle. However, CP functions of Apple stem pitting virus (ASPV) has not been comprehensively documented. This study aimed to characterize the functions of ASPV CP and any functional diversification caused by sequence diversity of six ASPV CP variants and studied their biological, serological, pathogenic and viral suppressor of RNA silencing (VSR) functions. METHODS:Six ASPV CP variants that have previously been shown to belong to different subgroups were selected here to study their diversity functions. Agrobacterium mediated infiltration (Agroinfiltration) was used to express YFP-ASPV-CPs in Nicotiana. benthamiana and infect Nicotiana. occidental with PVX-ASPV-CPs in. Confocal microscopy was used to detect YFP-ASPV-CPs florescence. CPs expressed in Escherichia coli BL21 (DE3) were induced by IPTG. RESULTS:In this study, we showed that recombinant CPs expressed in Escherichia coli BL21 (DE3) had different levels of serological reactivity to three anti-ASPV antibodies used to detect ASPV. Furthermore, fusion CPs with YFP (YFP-CPs) expressed in N. benthamiana cells differed in their ability to form aggregates. We also showed that ASPV isolates that harbour these CPs induced different biological symptoms on its herbaceous host N. occidentalis. At the same time, we found that all six CPs when expressed in PVX vector showed similar VSR activity and produced similar symptoms in N. occidentalis, despite their differences in amino acids. CONCLUSIONS:Different ASPV isolates induced different symptoms in N. occidentalis, however, ASPV CP variants expressed in PVX vector showed the same symptoms in N. occidentalis plants. Also, we showed that ASPV CP variants has the same level of VSR activity, but they have different abilities to aggregate in N. benthamiana.

Dataset Information

RNA sequencing data of tobacco inoculated with Apple stem pitting virus.

Publications

RNA sequencing data of tobacco inoculated with <i>Apple stem pitting virus</i>.

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