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First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China.

ABSTRACT: BACKGROUND:Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China. OBJECTIVE:The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle. METHODS:Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 CONCLUSIONS: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.

SUBMITTER: Yang Y

PROVIDER: S-EPMC7672823 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China.

Yang Yang Y Sun Yifan Y Li Qingliang Q Wu Yufeng Y Wang Deya D

Virology journal 20201118 1

<h4>Background</h4>Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China.<h4>Objective</h4>The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle.<h4>Methods</h4>Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential ...[more]

PMID: 33208142

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