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A Photoactivatable Small-Molecule Probe for the In Vivo Capture of Polyketide Intermediates.

ABSTRACT: A photolabile carba(dethia) malonyl N-acetylcysteamine derivative was devised and prepared for the trapping of biosynthetic polyketide intermediates following light activation. From the lasalocid?A polyketide assembly in a mutant strain of the soil bacterium Streptomyces lasaliensis, a previously undetected cyclised intermediate was identified and characterised, providing a new outlook on the timing of substrate processing.

SUBMITTER: Kilgour SL

PROVIDER: S-EPMC6972646 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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A Photoactivatable Small-Molecule Probe for the In Vivo Capture of Polyketide Intermediates.

Kilgour Samantha L SL Jenkins Robert R Tosin Manuela M

Chemistry (Weinheim an der Bergstrasse, Germany) 20191128 72

A photolabile carba(dethia) malonyl N-acetylcysteamine derivative was devised and prepared for the trapping of biosynthetic polyketide intermediates following light activation. From the lasalocid A polyketide assembly in a mutant strain of the soil bacterium Streptomyces lasaliensis, a previously undetected cyclised intermediate was identified and characterised, providing a new outlook on the timing of substrate processing. ...[more]

PMID: 31617624

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