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A Light-Activated Acyl Carrier Protein "Trap" for Intermediate Capture in Type?II Iterative Polyketide Biocatalysis.

ABSTRACT: A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type?II actinorhodin "minimal" polyketide synthase (PKS), a range of putative ACP-bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT-ICR-MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.

SUBMITTER: Kilgour SL

PROVIDER: S-EPMC6972679 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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A Light-Activated Acyl Carrier Protein "Trap" for Intermediate Capture in Type II Iterative Polyketide Biocatalysis.

Kilgour Samantha L SL Kilgour David P A DPA Prasongpholchai Panward P O'Connor Peter B PB Tosin Manuela M

Chemistry (Weinheim an der Bergstrasse, Germany) 20191206 72

A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin "minimal" polyketide synthase (PKS), a range of putative ACP-bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT ...[more]

PMID: 31596972

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