Ontology highlight
ABSTRACT:
SUBMITTER: Yan P
PROVIDER: S-EPMC6974442 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Frontiers in bioengineering and biotechnology 20200115
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as "Nimble Cloning" uses the restriction enzyme, <i>Sfi</i>I, in combination with the T5 exonuclease, to linearize the vector and generate 3'-overhangs simultaneously. Both PCR products and plasmids can be used for the cloning reaction in the ...[more]