Dataset Information

Antibodies raised against a Sunn bug (Eurygaster integriceps Put.) recombinant protease, rGHP3p2, can inhibit gluten-hydrolyzing activity.

ABSTRACT: Sunn pest or Sunn bug, Eurygaster integriceps Put., salivary gland proteases are responsible for the deterioration of wheat flour quality during dough mixing, resulting from gluten hydrolysis. These proteases are highly heterogeneous and show low sensitivity to most types of proteinaceous inhibitors, meaning that such inhibitors cannot be used to prevent gluten damage. The present study describes the generation of a specific peptide antibody, raised against the active center of the recombinant gluten-hydrolyzing protease (GHP3). The recombinant protein, encoding two repeats of the GHP3 sequence element involved in forming the S4 pocket and binding of substrate at position P4, was designed and expressed in Escherichia coli. The antibodies raised to this recombinant protein showed inhibitory activity against the GHP3 protease. The results indicate that it is possible to design specific antibodies to inhibit wheat-bug gluten-hydrolyzing proteases.

SUBMITTER: Dolgikh V

PROVIDER: S-EPMC6977494 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Antibodies raised against a Sunn bug (<i>Eurygaster integriceps</i> Put.) recombinant protease, rGHP3p2, can inhibit gluten-hydrolyzing activity.

Dolgikh Vyacheslav V Tsarev Alexander A Timofeev Sergey S Zhuravlyov Vladimir V Senderskiy Igor I Lovegrove Alison A Konarev Alexander A

Food science & nutrition 20191230 1

Sunn pest or Sunn bug, <i>Eurygaster integriceps</i> Put., salivary gland proteases are responsible for the deterioration of wheat flour quality during dough mixing, resulting from gluten hydrolysis. These proteases are highly heterogeneous and show low sensitivity to most types of proteinaceous inhibitors, meaning that such inhibitors cannot be used to prevent gluten damage. The present study describes the generation of a specific peptide antibody, raised against the active center of the recomb ...[more]

PMID: 31993194

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Project description:As a serious pest of wheat, the Sunn pest, Eurygaster integriceps Puton (Hem.: Scutelleridae), is prevalent in Iran. This pest belongs to univoltine species and tends to estivate and overwinter in high altitudes of nearby mountains as diapausing adults. The economic importance of the crop was attacked by this pest, i.e., wheat led the authors to study the physiological adaptations of these diapausing adults, that is, changes in the supercooling point (SCP), in the accumulation of cryoprotectants, and in the activities of the related enzymes in relation to diapause development. The mean SCP of the diapausing adults was found to be -8°C. The lowest SCP, i.e., approximately -11°C, was observed in the middle of diapause, October, when the highest cold hardiness was also interestingly recorded. This finding proposed that SCP depression could be a feasible cold-tolerance strategy for diapausing adults. The sugar content was high in the initiation and at the termination of diapause and was low during diapause maintenance. These sugar reserves were most likely utilized to be converted to glycogen and lipid during diapause maintenance as a survival strategy. The changes in the glycogen and lipid contents were inversely proportional to the changes in the total sugar content. The authors also found that the changes in the glycogen content were directly proportional to those in the low-molecular-weight carbohydrates (e.g., glycerol and trehalose) and in the diapause development. This finding underlined the role of the low-molecular-weight carbohydrates, such as the cryoprotectants, in enhancing the cold tolerance of the given insect. In this study, the diapause-associated changes in the activities of ?-amylases and proteases were also investigated. The results showed that the enzyme activities were related to diapause development and cold-tolerance enhancement. The highest enzyme activity was observed in September. Since the overwintering adults of the Sunn pest could not tolerate temperatures below their SCPs, they were grouped in the freeze-intolerant species.

Dataset Information

Antibodies raised against a Sunn bug (Eurygaster integriceps Put.) recombinant protease, rGHP3p2, can inhibit gluten-hydrolyzing activity.

Publications

Antibodies raised against a Sunn bug (<i>Eurygaster integriceps</i> Put.) recombinant protease, rGHP3p2, can inhibit gluten-hydrolyzing activity.

Similar Datasets

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