Ontology highlight
ABSTRACT:
SUBMITTER: Watkins EJ
PROVIDER: S-EPMC6980211 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Watkins Ella J EJ Almhjell Patrick J PJ Arnold Frances H FH
Chembiochem : a European journal of chemical biology 20191118 1-2
We report a simple, one-step enzymatic synthesis of the blue fluorescent noncanonical amino acid β-(1-azulenyl)-l-alanine (AzAla). By using an engineered tryptophan synthase β-subunit (TrpB), stereochemically pure AzAla can be synthesized at scale starting from commercially available azulene and l-serine. Mutation of a universally conserved catalytic glutamate in the active site to glycine has only a modest effect on native activity with indole but abolishes activity on azulene, suggesting that ...[more]