Ontology highlight
ABSTRACT:
SUBMITTER: DePasquale EAK
PROVIDER: S-EPMC6983270 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
DePasquale Erica A K EAK Schnell Daniel J DJ Van Camp Pieter-Jan PJ Valiente-Alandí Íñigo Í Blaxall Burns C BC Grimes H Leighton HL Singh Harinder H Salomonis Nathan N
Cell reports 20191101 6
Methods for single-cell RNA sequencing (scRNA-seq) have greatly advanced in recent years. While droplet- and well-based methods have increased the capture frequency of cells for scRNA-seq, these technologies readily produce technical artifacts, such as doublet cell captures. Doublets occurring between distinct cell types can appear as hybrid scRNA-seq profiles, but do not have distinct transcriptomes from individual cell states. We introduce DoubletDecon, an approach that detects doublets with a ...[more]