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Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes.


ABSTRACT: Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research has revealed that astrocytes are targets of ZIKV. However, the consequences of ZIKV infection, especially to this cell type, remain largely unknown, particularly considering integrative studies aiming to understand the crosstalk among key cellular mechanisms and fates involved in the neurotoxicity of the virus. Here, the consequences of ZIKV infection in iPSC-derived astrocytes are presented. Our results show ROS imbalance, mitochondrial defects and DNA breakage, which have been previously linked to neurological disorders. We have also detected glial reactivity, also present in mice and in post-mortem brains from infected neonates from the Northeast of Brazil. Given the role of glia in the developing brain, these findings may help to explain the observed effects in congenital Zika syndrome related to neuronal loss and motor deficit.

SUBMITTER: Ledur PF 

PROVIDER: S-EPMC6985105 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes.

Ledur Pítia Flores PF   Karmirian Karina K   Pedrosa Carolina da Silva Gouveia CDSG   Souza Leticia Rocha Quintino LRQ   Assis-de-Lemos Gabriela G   Martins Thiago Martino TM   Ferreira Jéssica de Cassia Cavalheiro Gomes JCCG   de Azevedo Reis Gabriel Ferreira GF   Silva Eduardo Santos ES   Silva Débora D   Salerno José Alexandre JA   Ornelas Isis Moraes IM   Devalle Sylvie S   Madeiro da Costa Rodrigo Furtado RF   Goto-Silva Livia L   Higa Luiza Mendonça LM   Melo Adriana A   Tanuri Amilcar A   Chimelli Leila L   Murata Marcos Massao MM   Garcez Patrícia Pestana PP   Filippi-Chiela Eduardo Cremonese EC   Galina Antonio A   Borges Helena Lobo HL   Rehen Stevens Kastrup SK  

Scientific reports 20200127 1


Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research has revealed that astrocytes are targets of ZIKV. However, the consequences of ZIKV infection, especially to this cell type, remain largely unknown, particularly considering integrative studies aiming to understand the crosstalk among key cellular mechanisms and fates involved in the neurotoxicity of the virus. Here, the consequences of ZIKV infection in iPSC-derived astrocytes are  ...[more]

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