Browse
Submit Data
Databases
API
Help

Dataset Information

17 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility.

ABSTRACT: To date, numerous biosensing platforms have been developed for assessing drug-induced cardiac toxicity by measuring the change in contractile force of cardiomyocytes. However, these low sensitivity, low-throughput, and time-consuming processes are severely limited in their real-time applications. Here, we propose a cantilever device integrated with a polydimethylsiloxane (PDMS)-encapsulated crack sensor to measure cardiac contractility. The crack sensor is chemically bonded to a PDMS thin layer that allows it to be operated very stably in culture media. The reliability of the proposed crack sensor has been improved dramatically compared to no encapsulation layer. The highly sensitive crack sensor continuously measures the cardiac contractility without changing its gauge factor for up to 26 days (>5 million heartbeats), while changes in contractile force induced by drugs are monitored using the crack sensor-integrated cantilever. Finally, experimental results are compared with those obtained via conventional optical methods to verify the feasibility of building a contraction-based drug-toxicity testing system.

SUBMITTER: Kim DS

PROVIDER: S-EPMC6985253 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Making Silicone Rubber Highly Resistant to Bacterial Attachment Using Thiol-ene Grafting.

Project description:Biomedical devices are indispensable in modern medicine yet offer surfaces that promote bacterial attachment and biofilm formation, resulting in acute and chronic healthcare-associated infections. We have developed a simple method to graft acrylates to silicone rubber, polydimethylsiloxane (PDMS), a commonly used device material that is often colonized by bacteria. We demonstrate a novel method whereby nontoxic bacteria attachment-resistant polymers can be readily grafted from and grafted to the surface using thiol-ene chemistry, substantially reducing bacterial colonization. With use of this approach, bacterial biofilm coverage can be reduced by 99% compared with standard PDMS in an in vitro assay. This grafting approach offers significant advantages over commonly used physisorbed coatings, especially in areas of high shear or mechanical stress. Furthermore, the approach is versatile such that the grafted material properties can be tailored for the desired final application.

| S-EPMC5138009 | biostudies-literature

A Highly Durable Rubber-Derived Lithium-Conducting Elastomer for Lithium Metal Batteries.

Project description:Elastomers offer attractive advantages over classical solid-state electrolytes in terms of ensuring stable interfacial contact and maintaining fatigue durability, but the low ionic conductivity obstructs their practical applications in long-life lithium metal batteries. In this work, rubber-derived lithium-conducting elastomer has been developed via sulfur vulcanization of nitrile butadiene rubber with a polymerizable ionic liquid to provide both high resilience and dramatically improved ionic conductivity. Owing to the chemically crosslinked network between rubber chains and ionic liquid fragments generated during vulcanization, the elastic lithium-conductor achieves high resilience of 0.92 MJ m-3 , superior cyclic durability of 1000 cycles at 50% strain, and high room-temperature ionic conductivity of 2.7 × 10-4 S cm-1 . Consequently, the corresponding solid-state lithium/LiFePO4 battery exhibits a high capacity of ≈146 mAh g-1 with a high capacity retention of 94.3% for up to 300 cycles.

| S-EPMC9165490 | biostudies-literature

Foot Plantar Pressure Measurement System Using Highly Sensitive Crack-Based Sensor.

Project description:Measuring the foot plantar pressure has the potential to be an important tool in many areas such as enhancing sports performance, diagnosing diseases, and rehabilitation. In general, the plantar pressure sensor should have robustness, durability, and high repeatability, as it should measure the pressure due to body weight. Here, we present a novel insole foot plantar pressure sensor using a highly sensitive crack-based strain sensor. The sensor is made of elastomer, stainless steel, a crack-based sensor, and a 3D-printed frame. Insoles are made of elastomer with Shore A 40, which is used as part of the sensor, to distribute the load to the sensor. The 3D-printed frame and stainless steel prevent breakage of the crack-based sensor and enable elastic behavior. The sensor response is highly repeatable and shows excellent durability even after 20,000 cycles. We show that the insole pressure sensor can be used as a real-time monitoring system using the pressure visualization program.

| S-EPMC6960515 | biostudies-literature

Highly Efficient Nanoscale Analysis of Plant Stomata and Cell Surface Using Polyaddition Silicone Rubber.

Project description:Stomata control gas exchange and water transpiration and are one of the most important physiological apparatuses in higher plants. The regulation of stomatal aperture is closely coordinated with photosynthesis, nutrient uptake, plant growth, development, and so on. With advances in scanning electron microscopy (SEM), high-resolution images of plant stomata and cell surfaces can be obtained from detached plant tissues. However, this method does not allow for rapid analysis of the dynamic variation of plant stomata and cell surfaces in situ under nondestructive conditions. In this study, we demonstrated a novel plant surface impression technique (PSIT, Silagum-Light as correction impression material based on A-silicones for all two-phase impression techniques) that allows for precise analysis of plant stomata aperture and cell surfaces. Using this method, we successfully monitored the dynamic variation of stomata and observed the nanoscale microstructure of soybean leaf trichomes and dragonfly wings. Additionally, compared with the analytical precision and the time used for preparing the observation samples between PSIT and traditional SEM, the results suggested that the analytical precision of PSIT was the same to traditional SEM, but the PSIT was more easy to operate. Thus, our results indicated that PSIT can be widely applied to the plant science field.

| S-EPMC6923247 | biostudies-literature

Highly Sensitive, Stretchable, and Robust Strain Sensor Based on Crack Propagation and Opening.

Project description:Soft and stretchable strain sensors have been attracting significant attention. However, the trade-off between the sensitivity (gauge factor) and the sensing range has been a major challenge. In this work, we report a soft stretchable resistive strain sensor with an unusual combination of high sensitivity, large sensing range, and high robustness. The sensor is made of a silver nanowire network embedded below the surface of an elastomeric matrix (e.g., poly(dimethylsiloxane)). Periodic mechanical cuts are applied to the top surface of the sensor, changing the current flow from uniformly across the sensor to along the conducting path defined by the open cracks. Both experiment and finite element analysis are conducted to study the effect of the slit depth, slit length, and pitch between the slits. The stretchable strain sensor can be integrated into wearable systems for monitoring physiological functions and body motions associated with different levels of strain, such as blood pressure and lower back health. Finally, a soft three-dimensional (3D) touch sensor that tracks both normal and shear stresses is developed for human-machine interfaces and tactile sensing for robotics.

| S-EPMC10403976 | biostudies-literature

Sideways and stable crack propagation in a silicone elastomer.

Project description:We have discovered a peculiar form of fracture that occurs in a highly stretchable silicone elastomer (Smooth-On Ecoflex 00-30). Under certain conditions, cracks propagate in a direction perpendicular to the initial precut and in the direction of the applied load. In other words, the crack deviates from the standard trajectory and instead propagates perpendicular to that trajectory. The crack arrests stably, and thus the material ahead of the crack front continues to sustain load, thereby enabling enormous stretchabilities. We call this phenomenon "sideways" and stable cracking. To explain this behavior, we first perform finite-element simulations that demonstrate a propensity for sideways cracking, even in an isotropic material. The simulations also highlight the importance of crack-tip blunting on the formation of sideways cracks. Next, we provide a hypothesis on the origin of sideways cracking that relates to microstructural anisotropy (in a nominally isotropic elastomer). To substantiate this hypothesis, we transversely prestretch samples to various extents before fracture testing, as to determine the influence of microstructural arrangement (chain alignment and strain-induced crystallization) on fracture energy. We also perform microstructural characterization that indicates that significant chain alignment and strain-induced crystallization indeed occur in this material upon stretching. We conclude by characterizing how a number of loading conditions, such as sample geometry and strain rate, affect this phenomenon. Overall, this paper provides fundamental mechanical insight into basic phenomena associated with fracture of elastomers.

| S-EPMC6511042 | biostudies-literature

Injectable silicone rubber for ocular implantation after evisceration.

Project description:ObjectiveTo investigate the usefulness of addition type liquid silicone rubber (ATLSR) as injectable implant after evisceration to maintain the eyeball volume in an animal experiment.MethodsTwelve adult New Zealand white rabbits were included. One eye of each rabbit was randomly selected for evisceration with the fellow eye as control. ATLSR was injected to fill the eyeball socket after evisceration. In vivo observation and photographs were performed up to 24 weeks post-op. Two rabbits were sacrificed respectively at post-operative week 1, 2, 4, 8, 12 and 24. After enucleation, the vertical, horizontal and sagittal diameters of the experimental eyeballs were measured and compared with the control eyes. Histopathological studies were performed to evaluate signs of inflammation.ResultsCornea remained clear throughout the observation period despite mild epithelial edema and neovascularization. Compared to the control eyes, the experimental eyes were significantly smaller in vertical diameter (17.00±1.17 vs. 17.54±1.11 mm, P<0.001), but larger in sagittal diameter (16.85±1.48 vs. 16.40±1.38 mm, P = 0.008), and had no significant difference in horizontal diameter (17.49±1.53 vs. 17.64±1.21 mm, P = 0.34). Postoperative inflammation was observed at one week after surgery, which peaked at 2-3 weeks, then regressed gradually. At week 12 and week 24, most of the inflammatory cells disappeared with some residual plasma cells and eosinophils.ConclusionInjectable addition type silicon rubber may be a good choice for ocular implantation after evisceration, maintaining eyeball volume and cosmetically satisfactory when compared to the fellow eye. Spontaneous regression of inflammation implied good biocompatibility for at least 24 weeks.

| S-EPMC5866100 | biostudies-literature

Realization of palladium-based optomechanical cantilever hydrogen sensor.

Project description:Hydrogen has attracted attention as an alternative fuel source and as an energy storage medium. However, the flammability of hydrogen at low concentrations makes it a safety concern. Thus, gas concentration measurements are a vital safety issue. Here we present the experimental realization of a palladium thin film cantilever optomechanical hydrogen gas sensor. We measured the instantaneous shape of the cantilever to nanometer-level accuracy using diffraction phase microscopy. Thus, we were able to quantify changes in the curvature of the cantilever as a function of hydrogen concentration and observed that the sensor's minimum detection limit was well below the 250 p.p.m. limit of our test equipment. Using the change in curvature versus the hydrogen curve for calibration, we accurately determined the hydrogen concentrations for a random sequence of exposures. In addition, we calculated the change in film stress as a function of hydrogen concentration and observed a greater sensitivity at lower concentrations.

| S-EPMC6445021 | biostudies-literature

Measuring molecular elasticity by atomic force microscope cantilever fluctuations.

Project description:In single-molecule mechanics experiments the molecular elasticity is usually measured from the deformation in response to a controlled applied force, e.g., via an atomic force microscope cantilever. We have tested the validity of an alternative method based on a recently developed theory. The concept is to measure the change in thermal fluctuations of the cantilever tip with and without its coupling to a rigid surface via the molecule. The new method was demonstrated by its application to the elasticity measurements of L- and P-selectin complexed with P-selectin glycoprotein ligand-1 or their respective antibodies, which showed values comparable to those measured from the slope of the force-extension curve. L- and P-selectin were found to behave as nearly linear springs capable of sustaining large forces and strains without sudden unfolding. The measured spring constants of approximately 4 and approximately 1 pN/nm for L- and P-selectin, respectively, suggest that a physiological force of approximately 100 pN would result in an approximately 200% strain for the respective selectins.

| S-EPMC1367073 | biostudies-literature

Magnetic properties of individual Co2FeGa Heusler nanoparticles studied at room temperature by a highly sensitive co-resonant cantilever sensor.

Project description:The investigation of properties of nanoparticles is an important task to pave the way for progress and new applications in many fields of research like biotechnology, medicine and magnetic storage techniques. The study of nanoparticles with ever decreasing size is a challenge for commonly employed methods and techniques. It requires increasingly complex measurement setups, often low temperatures and a size reduction of the respective sensors to achieve the necessary sensitivity and resolution. Here, we present results on how magnetic properties of individual nanoparticles can be measured at room temperature and with a conventional scanning force microscopy setup combined with a co-resonant cantilever magnetometry approach. We investigate individual Co2FeGa Heusler nanoparticles with diameters of the order of 35?nm encapsulated in carbon nanotubes. We observed, for the first time, magnetic switching of these nanoparticles in an external magnetic field by simple laser deflection detection. Furthermore, we were able to deduce magnetic properties of these nanoparticles which are in good agreement with previous results obtained with large nanoparticle ensembles in other experiments. In order to do this, we expand the analytical description of the frequency shift signal in cantilever magnetometry to a more general formulation, taking unaligned sensor oscillation directions with respect to the magnetic field into account.

| S-EPMC5566407 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data