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Removal of Cr(VI) by magnetic iron oxide nanoparticles synthesized from extracellular polymeric substances of chromium resistant acid-tolerant bacterium Lysinibacillus sphaericus RTA-01.

ABSTRACT: Background:Extracellular polymeric substances (EPS) from Cr(VI) resistant acid-tolerant biofilm forming bacterium (CrRAtBb) Lysinibacillus sphaericus RTA-01 was used for synthesis of magnetic iron oxide nanoparticles (MIONPs) in removal of Cr(VI). Methods:MIONPs synthesized in EPS matrix were characterized by UV-Vis, DLS, ATR-FTIR, XRD, FESEM, HRTEM and VSM. Primarily, the synthesis of MIONPs was established by the formation of black-colored precipitate through surface plasmon resonance (SPR) peak in between 330 and 450 nm. Results:The size of the spherical MIONPs with diameter range 13.75-106 nm was confirmed by DLS, XRD and FESEM analysis. HRTEM study confirmed the size of the MIONPs in the range of 10-65 nm. Moreover, the EDX and SAED confirmed the purity and polycrystalline nature of MIONPs. The ATR-FTIR peaks below 1000 cm-1 designated the synthesis of MIONPs. Also, the magnetic property of MIONPs was confirmed for separation from the aqueous solution. MIONPs were further checked for the adsorption of Cr(VI) with initial concentration range of 50-200 mg L-1. An adsorption isotherm and thermodynamic study were also carried out and the experimental data was best fitted in Langmuir isotherm model with maximum adsorption percent of 1052.63 mg g-1 of Cr(VI). Post interaction with Cr(VI), the surface characteristic of MIONPs in EPS matrix was evaluated by zeta potential, EDX, ATR-FTIR and XRD. Conclusion:This study ascertained the adsorption of Cr(VI) over EPS stabilized MIONPs whereas the zeta potential and XRD analysis confirmed the presence of reduced Cr(IV) on the adsorbent surface.

SUBMITTER: Kumar H

PROVIDER: S-EPMC6985332 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Removal of Cr(VI) by magnetic iron oxide nanoparticles synthesized from extracellular polymeric substances of chromium resistant acid-tolerant bacterium <i>Lysinibacillus sphaericus</i> RTA-01.

Kumar Himanshu H Sinha Swapan Kumar SK Goud Vaibhav V VV Das Surajit S

Journal of environmental health science & engineering 20191123 2

<h4>Background</h4>Extracellular polymeric substances (EPS) from Cr(VI) resistant acid-tolerant biofilm forming bacterium (CrRAtBb) <i>Lysinibacillus sphaericus</i> RTA-01 was used for synthesis of magnetic iron oxide nanoparticles (MIONPs) in removal of Cr(VI).<h4>Methods</h4>MIONPs synthesized in EPS matrix were characterized by UV-Vis, DLS, ATR-FTIR, XRD, FESEM, HRTEM and VSM. Primarily, the synthesis of MIONPs was established by the formation of black-colored precipitate through surface plas ...[more]

PMID: 32030170

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Project description:BackgroundEarly in the 1990s, it was recognized that Lysinibacillus sphaericus, one of the most popular and effective entomopathogenic bacteria, was a highly heterogeneous group. Many authors have even proposed it comprises more than one species, but the lack of phenotypic traits that guarantee an accurate differentiation has not allowed this issue to be clarified. Now that genomic technologies are rapidly advancing, it is possible to address the problem from a whole genome perspective, getting insights into the phylogeny, evolutive history and biology itself.ResultsThe genome of the Colombian strain L. sphaericus OT4b.49 was sequenced, assembled and annotated, obtaining 3 chromosomal contigs and no evidence of plasmids. Using these sequences and the 13 other L. sphaericus genomes available on the NCBI database, we carried out comparative genomic analyses that included whole genome alignments, searching for mobile elements, phylogenomic metrics (TETRA, ANI and in-silico DDH) and pan-genome assessments. The results support the hypothesis about this species as a very heterogeneous group. The entomopathogenic lineage is actually a single and independent species with 3728 core genes and 2153 accessory genes, whereas each non-toxic strain seems to be a separate species, though without a clear circumscription. Toxin-encoding genes, binA, B and mtx1, 2, 3 could be acquired via horizontal gene transfer in a single evolutionary event. The non-toxic strain OT4b.31 is the most related with the type strain KCTC 3346.ConclusionsThe current L. sphaericus is actually a sensu lato due to a sub-estimation of diversity accrued using traditional non-genomics based classification strategies. The toxic lineage is the most studied with regards to its larvicidal activity, which is a greatly conserved trait among these strains and thus, their differentiating feature. Further studies are needed in order to establish a univocal classification of the non-toxic strains that, according to our results, seem to be a paraphyletic group.

Dataset Information

Removal of Cr(VI) by magnetic iron oxide nanoparticles synthesized from extracellular polymeric substances of chromium resistant acid-tolerant bacterium Lysinibacillus sphaericus RTA-01.

Publications

Removal of Cr(VI) by magnetic iron oxide nanoparticles synthesized from extracellular polymeric substances of chromium resistant acid-tolerant bacterium <i>Lysinibacillus sphaericus</i> RTA-01.

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