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MicroRNA-21 affects mechanical force-induced midpalatal suture remodelling.


ABSTRACT:

Objectives

miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures.

Materials and methods

miR-21 deficient (miR-21-/- ) and wild-type (WT) mice were used to establish animal models by expanding the palatal sutures. Micro-CT, haematoxylin-eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, fluorescence labelling and immunohistochemistry were used to investigate the function of miR-21 in midpalatal suture bone remodelling. Besides, bone mesenchymal stem cells (BMSCs) derived from both miR-21-/- and WT mice were cultured. The MTT, CCK8, EdU analysis, transwell and wound healing test were used to assess the effects of miR-21 on the characteristics of cells.

Results

The expression of ALP was suppressed in miR-21-/- mice after expansion except 28 days. The expression of Ocn in WT mice was much higher than that of miR-21-/-  mice. Besides, with mechanical force, miR-21 deficiency downregulated the expression of Opg, upregulated the expression of Rankl, and induced more osteoclasts as TRAP staining showed. After injecting agomir-21  to miR-21-/- mice, the expression of Alp, Ocn and Opg/Rankl were rescued. In vitro, the experiments suggested that miR-21 deficiency reduced proliferation and migration ability of BMSCs.

Conclusions

The results showed that miR-21 deficiency reduced the rate of bone formation and prolonged the process of bone formation. miR-21 regulated the bone resorption and osteoclastogenesis by affecting the cell abilities of proliferation and migration.

SUBMITTER: Li M 

PROVIDER: S-EPMC6985676 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Publications

MicroRNA-21 affects mechanical force-induced midpalatal suture remodelling.

Li Mengying M   Zhang Zijie Z   Gu Xiuge X   Jin Ye Y   Feng Cheng C   Yang Shuangyan S   Wei Fulan F  

Cell proliferation 20191112 1


<h4>Objectives</h4>miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures.<h4>Materials and methods</h4>miR-21 deficient (miR-21<sup>-/-</sup> ) and wild-type (WT) mice were used to establish animal models by expanding the palatal sutures. Micro-CT, haematoxy  ...[more]

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