Ontology highlight
ABSTRACT:
SUBMITTER: Stanisic A
PROVIDER: S-EPMC6988596 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Stanišić Aleksa A Hüsken Annika A Kries Hajo H
Chemical science 20191003 44
Adenylation enzymes selecting substrates for ribosomal and nonribosomal protein and peptide biosynthesis have been popular targets of enzyme engineering. Previous standard assays for adenylation specificity have been cumbersome and failed to reflect the competition conditions inside a cell because they measure substrates one at a time. We have developed an adenylation assay based on hydroxamate quenching and LC-MS/MS detection of hydroxamate products testing dozens of competing amino acid substr ...[more]