Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics.

ABSTRACT: Oligonucleotide-conjugated antibodies have gained importance for their use in protein diagnostics. The possibility to transfer the readout signal from the protein to the DNA level with an oligonucleotide-conjugated antibody increased the sensitivity of protein assays by orders of magnitude and enabled new multiplexing strategies. A bottleneck in the generation of larger oligonucleotide-conjugated antibody panels is the low conjugation yield between antibodies and oligonucleotides, as well as the lack of product purification methods. In this study, we combined a non-site-directed antibody conjugation technique using copper-free click chemistry with ion-exchange chromatography to obtain purified single and double oligonucleotide-conjugated antibodies. We optimized the click conjugation react

SUBMITTER: Wiener J 

PROVIDER: S-EPMC6989672 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature