Ontology highlight
ABSTRACT:
SUBMITTER: Tan J
PROVIDER: S-EPMC6994485 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Nature communications 20200131 1
Base editors (BEs) are RNA-guided CRISPR-Cas-derived genome editing tools that induce single-nucleotide changes. The limitations of current BEs lie in their low precision (especially when multiple target nucleotides of the deaminase are present within the activity window) and their restriction to targets that are in proper distance from the PAM sequence. We have recently developed high-precision cytidine BEs by engineering CDA1 truncations and nCas9 fusions that predominantly edit nucleotide C<s ...[more]