Ontology highlight
ABSTRACT:
SUBMITTER: Sorkin R
PROVIDER: S-EPMC7002981 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Sorkin Raya R Marchetti Margherita M Logtenberg Emma E Piontek Melissa C MC Kerklingh Emma E Brand Guy G Voleti Rashmi R Rizo Josep J Roos Wouter H WH Groffen Alexander J AJ Wuite Gijs J L GJL
Biophysical journal 20191225 3
Synaptotagmin-1 (Syt1) is a calcium sensor protein that is critical for neurotransmission and is therefore extensively studied. Here, we use pairs of optically trapped beads coated with SNARE-free synthetic membranes to investigate Syt1-induced membrane remodeling. This activity is compared with that of Doc2b, which contains a conserved C<sub>2</sub>AB domain and induces membrane tethering and hemifusion in this cell-free model. We find that the soluble C<sub>2</sub>AB domain of Syt1 strongly af ...[more]