Ontology highlight
ABSTRACT:
SUBMITTER: Tao F
PROVIDER: S-EPMC7003095 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Tao Fang F Zhao Kai K Zhao Qianqian Q Xiang Fangzhi F Han Guomin G
G3 (Bethesda, Md.) 20200206 2
<i>Aspergillus flavus</i> is a fungus that produces aflatoxin B1, one of the most carcinogenic secondary metabolites. Understanding the regulation mechanism of aflatoxin biosynthesis in this fungus requires precise methods for genomic integration of mutant alleles. To avoid the disadvantage of DNA integration into the genome by non-homologous or ectopic recombination, we developed a novel strategy for site-specific integration of foreign DNA by using a carboxin-resistant <i>sdh2<sup>R</sup></i> ...[more]