Dataset Information

Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related A?42 Oligomers and Protect Synapses.

ABSTRACT: Impaired synaptic integrity and function due to accumulation of amyloid ?-protein (A?42) oligomers is thought to be a major contributor to cognitive decline in Alzheimer's disease (AD). However, the exact role of A?42 oligomers in synaptotoxicity and the ability of peripheral innate immune cells to rescue synapses remain poorly understood due to the metastable nature of oligomers. Here, we utilized photo-induced cross-linking to stabilize pure oligomers and study their effects vs. fibrils on synapses and protection by A?-phagocytic macrophages. We found that cortical neurons were more susceptible to A?42 oligomers than fibrils, triggering additional neuritic arborization retraction, functional alterations (hyperactivity and spike waveform), and loss of VGluT1- and PSD95-excitatory synapses. Co-culturing neurons with bone marrow-derived macrophages protected synapses against A?42 fibrils; moreover, immune activation with glatiramer acetate (GA) conferred further protection against oligomers. Mechanisms involved increased A?42 removal by macrophages, amplified by GA stimulation: fibrils were largely cleared through intracellular CD36/EEA1+-early endosomal proteolysis, while oligomers were primarily removed via extracellular/MMP-9 enzymatic degradation. In vivo studies in GA-immunized or CD115+-monocyte-grafted APPSWE/PS1?E9-transgenic mice followed by pre- and postsynaptic analyses of entorhinal cortex and hippocampal substructures corroborated our in vitro findings of macrophage-mediated synaptic preservation. Together, our data demonstrate that activated macrophages effectively clear A?42 oligomers and rescue VGluT1/PSD95 synapses, providing rationale for harnessing macrophages to treat AD.

SUBMITTER: Li S

PROVIDER: S-EPMC7005081 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related Aβ42 Oligomers and Protect Synapses.

Li Songlin S Hayden Eric Y EY Garcia Veronica J VJ Fuchs Dieu-Trang DT Sheyn Julia J Daley David A DA Rentsendorj Altan A Torbati Tania T Black Keith L KL Rutishauser Ueli U Teplow David B DB Koronyo Yosef Y Koronyo-Hamaoui Maya M

Frontiers in immunology 20200131

Impaired synaptic integrity and function due to accumulation of amyloid β-protein (Aβ42) oligomers is thought to be a major contributor to cognitive decline in Alzheimer's disease (AD). However, the exact role of Aβ42 oligomers in synaptotoxicity and the ability of peripheral innate immune cells to rescue synapses remain poorly understood due to the metastable nature of oligomers. Here, we utilized photo-induced cross-linking to stabilize pure oligomers and study their effe ...[more]

PMID: 32082319

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Dataset Information

Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related A?42 Oligomers and Protect Synapses.

Publications

Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related Aβ<sub>42</sub> Oligomers and Protect Synapses.

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